Journal: Journal of Musculoskeletal & Neuronal Interactions
Article Title: The mechanism of medial collateral ligament repair in knee osteoarthritis based on the TLR4/MyD88/NF-κB inflammatory signaling pathway
doi:
Figure Lengend Snippet: Histological changes of knee joint of 5-months-old SD rats under an optical microscope (HE staining x200). A: The Sham operation group. B: The model group. C: The repair group.
Article Snippet: Trizol (Sigma Company, USA); RNA reverse transcription kit (TOYOBO), SYBY® κGreen Real-time PCR Master Mi× (TOYOBO), and 5×Loading Buffer (TAkara Company, USA); DNA Marker (Shanghai Sangon Biotech Co., Ltd.); paraffin, paraformaldehyde, and hematoxylin-eosin (HE) staining reagent (Shanghai Beyotime Institute of Biotechnology), protein extraction kit and sodium-dodecyl-sulfate-polyacrylamide gel-electrophoresis (SDS-PAGE) kit ((Shanghai Beyotime Institute of Biotechnology); electrochemiluminescence (ECL) liquid (Applygen Technology Company); goat anti-rabbit immunoglobulin G (IgG)/horseradish peroxidase (HRP), goat anti-rabbit MyD88, TLR 4, and NF-κB (ab102890, ab16894, ab22048, and ab22146, Abcom Company, USA); TNF-α, IL-6, and IL-β enzyme-linked immuno-sorbent assay (ELISA) kits (Shanghai Beyotime Institute of Biotechnology).
Techniques: Microscopy, Staining